[PDF] Simple Chemical Extraction Method for DNA Isolation from Aspergillus fumigatus and Other Aspergillus Species | Semantic Scholar (2024)

An efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens, is developed, demonstrating that this method provides DNA of sufficiently high quality for molecular analyses.

Several methods are described that have been developed in the laboratory that allow the extremely rapid and inexpensive preparation of fungal genomic DNA.

The six extraction methods used to measure the recovery of DNA from two important fungal pathogens subjected to six DNA extraction methods produce markedly differing yields of fungal DNA and thus can significantly affect the results ofFungal PCR assays.

The DNA extracts obtained could be used directly without previous purification and quantification for RAPD analysis to differentiate cyclopiazonic acid producer from nonproducer mold strains, a procedure of great interest in food safety.

These processes of extraction by boiling and freeze-thawing processes without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.

A quick protocol for standardizing the procedure of species determination by sequencing of the elongation factor 1- and multiplex genetic chemotyping using the Tri12 gene, based on fungal growth on Miracloth tissue coupled with microwave extraction is proposed and statistically validated.

Aspergillosis is one of the important fungal infections, which is caused by Aspergillus fumigatus and less commonly by other AsperGillus species and is the second more expensive health problem on an affected flock basis.

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Three new methods for isolating denatured DNAs from filamentous fungi for dot-blot analysis are offered, including a rapid method for isolations from large numbers of small- to medium-scale cultures of filamentous bacteria and a nonenzymatic method that results in uniform signals for a variety of species.

The characteristics of the environmental fungal population explains the two main results obtained from the typing of the clinical isolates: the absence of a common strain responsible for an invasive aspergillosis outbreak results from the extreme diversity of theEnvironmental population of A. fumigatus in contact with the patients.

The genotypic relatedness between clinical and environmental isolates suggests that patients with invasive aspergillosis can be infected by strains originating from water or from air, and the genetic variability found between airborne and waterborne A. fumigatus strains might prove to be a powerful tool in understanding the transmission of invasive asenosis and in outbreak control.

Under transient selection as a pathogen, genes encoding alleles for enhanced virulence may not assort independently from microsatellite loci, and a dynamic equilibrium may exist between random recombination of loci in the natural environment and selection for virulence factors during host infection cycles.

This review summarizes the current knowledge on the biology, molecular genetics, and genomics of A. fumigatus and has a major impact on the development of novel strategies leading to the identification of antimycotic drugs.

Comparison of the peptidic sequences with other putative polypeptides of fungal LTR retrotransposons showed that Afut1 is a member of the gypsy group, the first report of a transposable element in A.fumigatus.

Analysis of transformants carrying the entire plasmid showed that up to 45% of integration had occurred at the pyrG locus, providing a tool to target defined genetic constructs at a specific locus in the A. fumigatus genome in order to study gene regulation and function.

The results demonstrate that, although functionally conserved, the mating pathway in C. neoformans has a unique organization.

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